Mean Size	Minimum pooled amount
<5 kb	150 ng
5-7 kb	200 ng
>7 kb	300 ng

Mean Size	Minimum per sample amount
<5 kb	150 ng
5-7 kb	200 ng
>7 kb	300 ng

Timings

Timing

Action	Time Spent
Render Time	31.6730 ms
Controller Time	0.0269 ms
Total Time	31.9679 ms

Template

Total Time	Engine	Count	Template	Content Type
31.6730 ms	kajiki	-	qbonline.templates.v2.service.pacbio_sequencing	text/html
31.5590 ms	kajiki	1	qbonline.templates.v2.service.pacbio_sequencing

Profiling

Calls	Total	Percall	Cumu	CumuPer	Func
1	0.003	0.0030	0.007	0.0070	<tg/configurator/components/dispatch.py:113(_call_controller)>
1	0.004	0.0040	0.004	0.0040	<qbonline/controllers/v2/service.py:60(pacbio_sequencing)>
1	0.001	0.0010	0.001	0.0010	{method 'disable' of '_lsprof.Profiler' objects}

Request

GET

Nothing here.

POST

Nothing here.

Cookies

Nothing here.

Headers

Name	Value
SERVER_SOFTWARE	'WSGIServer/0.1 Python/2.7.18'
SCRIPT_NAME	''
REQUEST_METHOD	'GET'
SERVER_PROTOCOL	'HTTP/1.0'
SERVER_PORT	'8088'
HTTP_HOST	'18.208.226.9'
HTTP_ACCEPT	'/'
HTTP_X_FORWARDED_FOR	'3.149.249.68'
PATH_INFO	'/service/pacbio_sequencing'
QUERY_STRING	''
HTTP_X_REAL_IP	'3.149.249.68'
HTTP_USER_AGENT	'Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)'
HTTP_CONNECTION	'close'
REMOTE_ADDR	'172.18.0.1'
SERVER_NAME	'c380b01a81ae'
HTTP_X_FORWARDED_HOST	'turbo.quintarabio.com'
CONTENT_TYPE	'text/plain'
REMOTE_HOST	'ip-172-18-0-1.ec2.internal'
HTTP_ACCEPT_ENCODING	'gzip, br, zstd, deflate'

Request Attributes

Name	Value
response	"{'_app_iter': [''], '_status': '200 OK', '_headers': ResponseHeaders([('Content-Length', '0'), ('Pragma', 'no-cache'), ('Cache-Control', 'no-cache'), ('Content-Type', 'text/html; charset=utf-8')]), 'conditional_response': False, '_headerlist': [('Content-Length', '0'), ('Pragma', 'no-cache'), ('Cache-Control', 'no-cache'), ('Content-Type', 'text/html; charset=utf-8')]}"
identity	None

Environ

Name	Value
beaker.cache	<beaker.cache.CacheManager object at 0x7f2be13b2450>
SERVER_SOFTWARE	'WSGIServer/0.1 Python/2.7.18'
SCRIPT_NAME	''
REQUEST_METHOD	'GET'
SERVER_PROTOCOL	'HTTP/1.0'
HOME	'/root'
repoze.who.plugins	{'cookie': <AuthTktCookiePlugin 139826439143952>, 'form': <FastFormPlugin:/login_handler 139826439183440>, 'main_identifier': <FastFormPlugin:/login_handler 139826439183440>, 'tgappauth': <tg.configuration.auth.metadata._AuthMetadataAuthenticator object at 0x7f2be13b2610>}
CONTENT_LENGTH	''
SERVER_PORT	'8088'
PYTHONPATH	'/home/quintara/tg2env/lib/python2.7/site-packages:'
HTTP_HOST	'18.208.226.9'
beaker.session	{'_id': '3c2bc07d8ae04143ae005275f86c31bc', '_domain': None, '_path': '/'}
HTTP_ACCEPT	'/'
wsgi.version	(1, 0)
HTTP_X_FORWARDED_FOR	'3.149.249.68'
wsgi.errors	<open file '<stderr>', mode 'w' at 0x7f2be999d270>
wsgi.multiprocess	False
wsgi.run_once	False
tg.locals	<tg.wsgiapp.RequestLocals object at 0x7f2bb05e31d0>
HOSTNAME	'c380b01a81ae'
webob._parsed_query_vars	(GET([]), '')
webob.adhoc_attrs	{'identity': None}
repoze.who.api	<repoze.who.api.API object at 0x7f2bafdec410>
PATH_INFO	'/service/pacbio_sequencing'
beaker.get_session	<bound method SessionApplicationWrapper._get_session of <tg.appwrappers.session.SessionApplicationWrapper object at 0x7f2be13a3410>>
QUERY_STRING	''
PATH	'/home/quintara/tg2env/bin:/home/quintara/.local/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin'
HTTP_X_REAL_IP	'3.149.249.68'
HTTP_USER_AGENT	'Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)'
HTTP_CONNECTION	'close'
paste.registry	<tg.support.registry.Registry object at 0x7f2bafdec210>
REMOTE_ADDR	'172.18.0.1'
wsgi.url_scheme	'http'
wsgi.input	<socket._fileobject object at 0x7f2bbe93fd50>
wsgi.multithread	True
repoze.who.logger	<logging.Logger object at 0x7f2be3a25fd0>
SERVER_NAME	'c380b01a81ae'
GATEWAY_INTERFACE	'CGI/1.1'
HTTP_X_FORWARDED_HOST	'turbo.quintarabio.com'
PYTHONHOME	'/home/quintara/tg2env'
paste.cookies	(<SimpleCookie: >, '')
PWD	'/home/quintara/QBonline'
CONTENT_TYPE	'text/plain'
wsgi.file_wrapper	<class wsgiref.util.FileWrapper at 0x7f2be22800c0>
REMOTE_HOST	'ip-172-18-0-1.ec2.internal'
HTTP_ACCEPT_ENCODING	'gzip, br, zstd, deflate'
webob._parsed_cookies	({}, '')

SQLAlchemy

No queries in executed by the controller.

Controllers

Mounted Controllers

Path	Controller	Exposed Methods
/	<class 'qbonline.controllers.root.RootController'>	manage_permission_only, index, about, sample_prep, post_login, ordering, support, ngsdata, auth, post_logout, career, contact, environ, services, _default, login
/dnaprep	<class 'qbonline.controllers.v2.dnaprep.DNAPrepController'>	index, one
/oligo	<class 'qbonline.controllers.v2.oligo.OligoController'>	index, search, coligo, detail, coligo_preview, odetail, coligo_order, ohistory, packingSlipDetail, internalhistory, plasmid_dnaprep, history
/_debugbar	<class 'tgext.debugbar.controller.DebugBarController'>	perform_ming, perform_sql
/_debugbar/statics	<class 'tgext.debugbar.controller.StaticsController'>	_default
/cloning	<class 'qbonline.controllers.v2.cloning.CloningController'>	index, one
/dropbox	<class 'qbonline.controllers.v2.dropbox.DropboxController'>	dropbox_customer, dropboxManage
/accounting	<class 'qbonline.controllers.v2.accounting.AccountingController'>	cc_payment, applyPayment, blanketpo_sum, index, auto_bill_old, plookup, removeInvoices, npar_report_old, prepaid_1, print_and_mail, reviewAction, billcc, auto_review_old, billAction, cc_npar, printAndMailAction, ilookup, paymentAction, prepaid, manual_email, payment, calc_revenue, npar_report, processed_orders, auto_bill, prepaids, updateInvoiceInfo, billupdate, auto_review
/labscan	<class 'qbonline.controllers.v2.labscan.LabScanController'>	plate, whoisatwork, index, barcode, order, monthly_hours, sel, scan1, edit_timecard
/billing	<class 'qbonline.controllers.v2.billing.BillingController'>	billStatement, infoneed, download_invoices, editInvoiceOrder, agingDetails, fill_institution, invDetails, invoices_nopo, neverBilledSummary, notbill, index, neverBilledDetails, billOutstandingOrders, get_sales_balance, packingSlipSummary, invoice_pdf_upload, paymentSummary, quoteManage, account_balance, billgroupManage, collection, showInvoice, startBilling, editInvoiceStatus, paymentInvoiceSummary, packingslip, agingSummary, new_invoice
/pickup	<class 'qbonline.controllers.v2.pickup.PickupController'>	index
/sradmin	<class 'qbonline.controllers.v2.sradmin.SRAdminController'>	indext
/sradmin/billgroup	<class 'qbonline.controllers.v2.sradmin.BillGroupController'>	edit
/sradmin/customerrequest	<class 'qbonline.controllers.v2.sradmin.CustomerRequestController'>	edit
/sradmin/institution	<class 'qbonline.controllers.v2.sradmin.InstitutionController'>	edit
/sradmin/po	<class 'qbonline.controllers.v2.sradmin.POController'>	edit, new, pick_billgroup
/sradmin/invoice	<class 'qbonline.controllers.v2.sradmin.InvoiceController'>	edit
/sradmin/payment	<class 'qbonline.controllers.v2.sradmin.PaymentController'>	edit
/sradmin/quotation	<class 'qbonline.controllers.v2.sradmin.QuotationController'>	edit
/store	<class 'qbonline.controllers.v2.store.OnlineStoreController'>	index, save_b_address, shipping, get_address, confirm, placeorder, summary, shipping_billing_info_2, cart, pick_products, get_saved_address, pick_product_1, save_billgroup_address, recordorder, delete_address, q_maxseq, payment, save_s_address, payment_type
/bd	<class 'qbonline.controllers.v2.business.BusinessController'>	index, creport, crtable, delete
/plate	<class 'qbonline.controllers.v2.plate.PlateController'>	index, upload_ss, ss, review, update, edit, pdf, data, delete2
/quote	<class 'qbonline.controllers.v2.quote.QuoteController'>	seq, quote, pick_products, lookup, pick_product_1, save
/clone	<class 'qbonline.controllers.v2.clone.CloneController'>	index, all, cnew, create_download, confirm, twist1, twist_update, update, calljson, generate_quote, twist, packingslip, upload, dump_orders, new, call, preview, qclones, order1
/qcorder	<class 'qbonline.controllers.v2.qcorder.QCOrderController'>	seqorder, qcupdate
/user	<class 'qbonline.controllers.v2.user.UserController'>	clookup, myqb, pwreset, customer_lab_edit, get_report, download, billgroup_edit, dropbox_lookup, user_edit_2_old, index, download_from_email, save_billgroup_info, cancelo, group_lookup, lookup, lview_i_d, name_lookup, lview_d, customer_summary, lview_i, billgroup_address_edit, lview_m, lview_o, lview_p, lookup_user, order_lookup, prepaid_balance_detail, myAccount, user_edit_2, user_edit_3, prepaid_balance, register, myorder, download_r, lview, updateNickName, save_billgroup_address
/qbtools	<class 'qbonline.controllers.v2.qbtools.QbToolsController'>	feedback, reverse_complement, submit_feedback, translate, primer_check, findPrimer
/corder	<class 'qbonline.controllers.v2.corder.SeqOrderController'>	qorder2, c_seq_f2_json, osu, plasmid_dnaprep_c, download, prepaid_order_detail, plsexpress, qborder, index, c_seq_f0, c_seq_f1, c_seq_f2, c_seq_f3, c_file_1, clone_detail, c_seq_p_f2, c_seq_st, other_order_detail, cplasmid_dnaprep, qorder, orderUpdate, restriction_enzymes, ampseq_f1, c_seq_p, ampseq_f2, sequencing, c_seq_p_f3, order1, plasmid_dnaprep_st, c_file, myorder, odetail, view_trace, ampseq_st, plsexpc, plsexpst
/qbadmin	<class 'qbonline.controllers.v2.qbadmin.QbAdminController'>	manageGroup, weeklyreports, mtreport, weeklyreport_1, viewlog, index, ppts, plasmids, add2collect, plasmid_action, weeklyreport_c, remove, weekly, daily_plate, weeklyreport_n, add2collect_save
/service	<class 'qbonline.controllers.v2.service.ServiceController'>	plasmidexpress, ampseq, dnaprep, request_samples, cloning, sanger, celllineedit, plsexpress, index, ngs, cloning_services, oligo, sanger_sequencing, ngs_services, pacbio_sequencing, amp, cssdnaq, cssdna, dnaprep_services, request_info, covid, gene, oligo_synthesis
/project	<class 'qbonline.controllers.v2.project.ProjectController'>	index, one230, update, ngs, download, plsexps_o
/dashboard	<class 'qbonline.controllers.v2.dashboard.DashboardController'>	pickupRoute, do, _email, adminCustomerUpdate, billgroupManage, copyToClipboard, index, get_user_json, nanopore, findOrders, checkout, ssValidator, primer, orderTrans, _date

Logging

Log Messages

Level	Time	Message	Location

Inventing

Inventing Mode

Invending mode permits to see on the fly the result of your changes by automatically reloading the web page whenever your python code or the page template changes.

It is meant as a way to improve developer experience when experimenting with changes to the source code, just put your browser and your test editor one near the other and enable inventing mode to start experimenting.

Turn On

To permanently enable inventing mode when in debug add debugbar.inventing=True option to your application configuration.

PacBio Sequencing Service

Single-Molecule, Read-Time (SMRT) Sequencing

Highly Acurrate Long Reads

Quintara offers a complete package of PacBio sequencing services, from assay design and library prep to sequencing reactions and data analysis.

WORKFLOW

1. Sample Submission

2. Library Preparation

3. Sequencing

4. Data Analysis

SAMPLE PREPARATION

PCR-barcoded samples

Adapter-barcoded samples

Free sample pick-up available

REQUEST MORE INFORMATION

Timing

Template

Profiling

GET

POST

Cookies

Headers

Request Attributes

Environ

Mounted Controllers

Log Messages

Inventing Mode